|
KMID : 1137020180290010017
|
|
Journal of Gynecologic Oncology
2018 Volume.29 No. 1 p.17 ~ p.17
|
|
|
The feasibility of detecting endometrial and ovarian cancer using DNA methylation biomarkers in cervical scrapings
|
|
Chang Cheng Chang
Wang Hui Chen
Liao Yu Ping
Chen Yu Chih
Weng Yu Chun
Yu Mu Hsien
Lai Hung Cheng
|
|
|
Abstract
|
|
|
Objective: We hypothesized that DNA methylation of development-related genes may occur in endometrial cancer (EC)/ovarian cancer (OC) and may be detected in cervical scrapings.
Methods: We tested methylation status by quantitative methylation-specific polymerase chain reaction for 14 genes in DNA pools of endometrial and OC tissues. Tissues of EC/normal endometrium, OC/normal ovary, were verified in training set using cervical scrapings of 10 EC/10 OC patients and 10 controls, and further validated in the testing set using independent cervical scrapings in 30 EC/30 OC patients and 30 controls. We generated cutoff values of methylation index (M-index) from cervical scrapings to distinguish between cancer patients and control. Sensitivity/specificity of DNA methylation biomarkers in detecting EC and OC was calculated.
Results: Of 14 genes, 4 (PTGDR, HS3ST2, POU4F3, MAGI2) showed hypermethylation in EC and OC tissues, and were verified in training set. POU4F3 and MAGI2 exhibited hypermethylation in training set were validated in independent cases. The mean M-index of POU4F3 is 78.28 in EC and 20.36 in OC, which are higher than that in controls (6.59; p<0.001 and p=0.100, respectively), and that of MAGI2 is 246.0 in EC and 12.2 in OC, which is significantly higher that than in controls (2.85; p<0.001 and p=0.480, respectively). Sensitivity and specificity of POU4F3/MAGI2 were 83%?90% and 69%?75% for detection of EC, and 61% and 62%?69% for the detection of OC.
Conclusion: The findings demonstrate the potential of EC/OC detection through testing for DNA methylation in cervical scrapings.
|
|
|
KEYWORD
|
|
|
Endometrial Neoplasms, Ovarian Neoplasms, DNA Methylation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
    
|
|